deoD [2019-08-05 09:12:48]

purine nucleoside phosphorylase

locus: BSU_19630

pI: 4.98

mw: 25.23 kDa

protein length: 233 aa

gene length: 702 bp

function: purine salvage and interconversion

product: purine nucleoside phosphorylase

essential: no

ec: 2.4.2.1

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.3|Purine salvage and interconversion]

Gene

Coordinates: 2,135,470 2,136,171

The protein

Catalyzed reaction/ biological activity

• Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate (according to Swiss-Prot)

Protein family

• PNP/UDP phosphorylase family (with [protein|5B21D1E73A65B13CE4DDA32ABC71EFA54E8D1D8F|MtnN], according to UniProt)

Structure

• [PDB|4D8V] [Pubmed|22957058]

Expression and Regulation

Operons

• genes: [gene|3075327DBE55C4F628171AEE248CAED5870693FA|deoD]-[gene|9A22825FD67B6A205807433D0B4908F86E5AB343|ldcB]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE19630 ([gene|3075327DBE55C4F628171AEE248CAED5870693FA|deoD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCATATCCTCCTGTAA, downstream forward: _UP4_TAAAATATATCAAGAGGCGT
• BKK19630 ([gene|3075327DBE55C4F628171AEE248CAED5870693FA|deoD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCATATCCTCCTGTAA, downstream forward: _UP4_TAAAATATATCAAGAGGCGT

References

• 21424839,16511068,22957058,22383849